Habitat. Tetanus is an acute, often fatal, disease caused by an exotoxin produced by the bacterium Clostridium tetani. Endospore stain of Clostridium botulinum (see Fig. The muscle stiffness usually involves the jaw (lockjaw) and neck and then becomes generalized. Clostridium, genus of rod-shaped, usually gram-positive bacteria, members of which are found in soil, water, and the intestinal tracts of humans and other animals. 11. As characteristic of all bacteria, C. tetani bacteria are single-celled and do not contain any membrane-bound organelles, such as a nucleus. Clostridium tetani is a moderately-sized gram-positive, endospore-producing bacillus. Shape: Spore-forming Rod. C. tetani's appearance on gram stain is said to resemble tennis rackets or drumsticks. Clostridium perfringens is large rectangular gram-positive bacilli with rounded or truncated ends. 16 Although classified as a Gram-positive organism, it may stain variably, especially in tissue or older cultures. Diseases. Clostridium tetani is an anaerobic pathogenic bacterium that is primarily found in soil and animal intestinal tracts. Motile with a peritrichous arrangement of flagella. Vegetative cells are rod shaped, pleomorphic, and occur in pairs or short chains Footnote 1. CHARACTERISTICS: Clostridium tetani is a motile, anaerobic, spore forming bacteria (terminal spores with drum stick appearance) Footnote 1, Footnote 2. Shapes and Arrangements. Dormant cells are highly resistant to heat, desiccation, and toxic chemicals Clostridium tetani is the etiological agent of tetanus, Clostridium botulinum is the etiological agent of botulism, and Clostridium perfringens is one of the etiological agent of gas gangrene. A: B: C: IV. Experiment (dry lab) ... Neisseria meningitidis: Mycobacterium leprae: Clostridium tetani: Clostridium botulinum: Bacillus anthracis: Proprionibacterium acnes: Salmonella enteritidis: V. Gram Staining. Tetanus is contracted through contact between spores of C. tetani and an … Produce round, terminal endospores that give the bacterium a "tennis-racquet" appearance. Experiment (dry lab) Record the cell shape. It is capsulated, non-motile and non-flagellated pathogenic bacteria. Found in nature as spores in soil or parasitising the gastrointestinal tracts of animals, these bacteria cause serious toxicity in humans. It is characterized by generalized rigidity and convulsive spasms of skeletal muscles. Observe the demonstration slide of Clostridium tetani. Colonizes the intestinal tract in humans and animals. Clostridium tetani is a slender, obligate anaerobic bacillus measuring 0.5–1.7 µm by 2.1–18.1 µm. Most species grow only in the complete absence of oxygen. Organism. A single, terminal spore is present at the end of each rod, giving a "Tennis Racket" like appearance: Transmission; C. tetani spores are common in the soil and may exist on sharp objects on the ground like rusty nails. 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